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On-Line 24/7!

24-Hour TEST Results

for Steam Sterilizers!

To view your sterilizer test results on-line, click the link above, enter your Login and password. Copy your report or print!

BACILLUS ATROPHAEUS SPORES

Biological indicators are the only process indicators that directly monitor the lethality of a given sterilization process.  Spores used to monitor a sterilization process have demonstrated resistance to the sterilizing agent and are more resistant than the bioburden found on medical devices.  Bacillus atrophaeus spores are used to monitor EO Gas and dry heat, and Geobacillus stearothermophilus spores are used to monitor steam sterilization, hydrogen peroxide gas plasma, and liquid peracetic acid sterilizers. Geobacillus stearothermophilus spores are incubated at 55-60C, and Bacillus atrophaeus spores are incubated at 35-37C. 

Weekly Testing is minimum:

Steam and low temperature sterilizers (e.g., hydrogen peroxide gas plasma, peracetic acid) should be monitored at least weekly with the appropriate commercial preparation of spores.  If a sterilizer is used frequently (e.g., several loads per day), daily use of biological indicators allows earlier discovery of equipment malfunctions or procedural errors and thus minimizes the extent of patient surveillance and product recall needed in the event of a positive biological indicator.  Each load should be monitored if it contains implantable objects.  If feasible, implantable items should not be used until the results of spore tests are known to be negative.  See also www.cdc.gov/oralhealth/infectioncontrol/faq/sterilization_monitoring.htm

The excellent microbicidal activity of ETO sterilizers has been demonstrated and proven in several studies.  ETO inactivates all microorganisms although bacterial spores (especially Bacillus atrophaeus) are more resistant than other microorganisms.  For this reason Bacillus atrophaeus spores is the recommended biological indicator.  Also, Bacillus atrophaeus spores should be used to monitor the sterilization process for dry heat because they are more resistant to dry heat than are Geobacillus stearothermophilus spores.  The primary lethal process is considered to be oxidation of cell constituents. 

ATS, Inc. facilitates high quality and stringent laboratory standards with our Mail-In Services.  We provide a dual-spore strip inoculated with both spores, Geobacillus stearothermophilus and Bacillus atrophaeus spores, allowing users to spore test all sterilizers used in healthcare facilities.  ATS also has a 48-hour read-out biological indicator for performing In-Office Testing.

DID YOU KNOW?

       Autoclave Testing Service, Inc. is one of the first companies nationwide to offer a Mail-In sterilizer monitoring service using biological indicators.  Serving the healthcare industry throughout the United States and Canada since 1986, we have thousands of satisfied customers.

       We have on-line test results allowing you to access your results at your convenience!  Your Sterilizer Test Report (STR) is posted on-line upon completion of culturing, offering minimum turn-around time.

       We have 24-hour test results for Steam sterilizers!

       We have a comprehensive Glossary of Terms related to the sterile processing area (scroll below to view the Glossary).  This is invaluable information, assisting you with this important area of your facility.

ATS, Inc. now stocks sterilization pouches, sterilizer cleaners, chemical indicator products, sterilizers (new and used), ultrasonic cleaners, water distillers, and more!  (see Supplies).

 

GLOSSARY OF TERMS USED:

 

Ask us about: Daily Autoclave Testing Requirements and Recommendations in Ontario, Canada